Transgenic non-human mammals
Abstract
A transgenic non-human eukaryotic animal whose germ cells and somatic cells contain an activated oncogene sequence introduced into the animal, or an ancestor of the animal, at an embryonic stage.
Claims
1. A transgenic non-human mammal all of whose germ cells and somatic cells contain a recombinant activated oncogene sequence introduced into said mammal, or an ancestor of said mammal, at an embryonic stage.
2. The mammal of claim 1, a chromosome of said mammal including an endogenous coding sequence substantially the same as a coding sequence of said oncogene sequence.
3. The mammal of claim 2, said oncogene sequence being integrated into a chromosome of said mammal at a site different from the location of said endogenous coding sequence.
4. The mammal of claim 2 wherein transcription of said oncogene sequence is under the control of a promoter sequence different from the promoter sequence controlling the transcription of said endogenous coding sequence.
5. The mammal of claim 4 wherein said promoter sequence controlling transcription of said oncogene sequence is inducible.
6. The mammal of claim 1 wherein said oncogene sequence comprises a coding sequence of a c-myc gene.
7. The mammal of claim 1 wherein transcription of said oncogene sequence is under the control of a viral promoter sequence.
8. The mammal of claim 7 wherein said viral promoter sequence comprises a sequence of an MMTV promoter.
9. The mammal of claim 7 wherein said viral promoter sequence comprises a sequence of an RSV promoter.
10. The mammal of claim 1 wherein transcription of said oncogene sequence is under the control of a synthetic promoter sequence.
11. The mammal of claim 1, said mammal being a rodent.
12. The mammal of claim 11, said rodent being a mouse.
Cabinet Chaillot